Impact of population and laboratory methods on DNA yield and variability
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چکیده
Total DNA yield from Oragene®/saliva samples† can vary due to intraand inter-donor variability, as well as the DNA extraction and quantification methods chosen. A comparative analysis of total DNA yield from peer-reviewed publications and in-house experiments is presented in this white paper to highlight the impact of population and laboratory method selection on final interpretation of data. Using comparative data, the average DNA yield from 2 mL of saliva extracted with prepIT®•L2P is 56 μg when quantified using fluorescence, and the average total nucleic acid yield is 129 μg when quantified by UV spectroscopy.
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